RNA Isolation from Adipose Tissue: An Optimized Procedure for High RNA Yield and Integrity
نویسندگان
چکیده
منابع مشابه
High Quality RNA Isolation from Leaf, Shell, Root Tissues and Callus of Hazelnut (Corylus avellana L.)
Extraction of high quality RNA is a critical step in molecular genetics studies. Hazelnut is one of the most important nuts plants in the world. The presence of the taxol and other taxanes in hazelnut plant necessitates explaining their biosynthesis pathway and identifying the candidate genes. Therefore, an easy and practical method is necessary for RNA extraction from hazelnuts. Hazelnut has h...
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In this study, we aimed to optimize the isolation procedure of circulating RNA from large volumes of plasma. Simultaneously, the stability and integrity of RNA from plasma samples were examined. To investigate the isolation of circulating RNA, reverse transcription-PCR analysis in combination with capillary electrophoresis was used. The presence of amplifiable RNA in plasma from healthy volunte...
متن کاملIsolation of high-quality RNA from a wide range of woody plants
Isolation of high-quality RNA is one of the most crucial methods in molecular biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody tissues, large amounts of polysaccharides, polyphenols and other secondary metabolites. Here we present a suitable protocol for RNA isolation from a wide range of woody plants that includes eight gymnosperms and four ...
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ژورنال
عنوان ژورنال: Laboratory Medicine
سال: 2010
ISSN: 0007-5027,1943-7730
DOI: 10.1309/lmfsbpuoa19mh5bv